Topical skin care formulations

ABSTRACT

Disclosed is a topical skin care composition comprising: (a) a combination of the following extracts:  Malpighia punicifolia  (acerola) extract;  Argania spinosa  (argan) extract;  Myrciaria dubia  (camu camu) extract;  Punica granatum  (pomegrannate) extract;  Pinus sylvestris  extract;  Terminalia ferdinandiana  (kakadu plum) extract;  Linum usitatissimum  (linseed) extract;  Ribes nigrum  (black current) extract;  Secale cereale  (rye) extract; algae extract; and yeast extract; and (b) a dermatologically acceptable vehicle.

CROSS REFERENCE TO RELATED APPLICATIONS

This application claims the benefit of U.S. Provisional Application Ser.No. 61/166,139, filed, Apr. 2, 2009, and U.S. Provisional ApplicationSer. No. 61/164,802, filed Mar. 30, 2009. The contents of theseapplications are incorporated by reference.

BACKGROUND OF THE INVENTION

A. Field of the Invention

The present invention relates generally to compositions that can be usedto improve the skin's visual appearance. In particular, the presentinvention concerns topical skin care compositions that include Arganiaspinosa (argan) seed kernel extract, Secale cereale (rye) seed extract,Linum usitatissimum (linseed) seed extract, Malpighia punicifolia(acerola) fruit extract, Terminalia ferdinandiana (kakadu plum) fruitextract, Myrciaria dubia (camu camu) fruit extract, Punica granatum(pomegrannate) fruit extract or sterols, Pinus sylvestris bark extract,Ribes nigrum (black currant) leaf extract, palmitoyl oligopeptide,palmitoyl tetrapeptide-7, or adenosine, or any combination thereof.

B. Description of Related Art

With aging, chronic exposure to adverse environmental factors, ormalnutrition, the visual appearance, physical properties, andphysiological functions of skin can change in ways that are consideredvisually undesirable. The most notable and obvious changes include thedevelopment of fine lines and wrinkles, loss of elasticity, increasedsagging, loss of firmness, loss of color evenness or tone, coarsesurface texture, and mottled pigmentation. Less obvious, but measurablechanges which occur as skin ages or endures chronic environmental insultinclude a general reduction in cellular and tissue vitality, reductionin cell replication rates, reduced cutaneous blood flow, reducedmoisture content, accumulated errors in structure and function,alterations in the normal regulation of common biochemical pathways, anda reduction in the skin's ability to remodel and repair itself. Many ofthe alterations in appearance and function of the skin are caused bychanges in the outer epidermal layer of the skin, while others arecaused by changes in the lower dermis.

For most of the existing skin treatment options that are available,there is a period of time, up to several weeks or months, during whichthe skin becomes irritated and after which tolerance sets in and thesymptoms of irritation may decrease and/or cease.

SUMMARY OF THE INVENTION

The present invention overcomes deficiencies in the art by providingcompositions that can be used in skin treatment applications. Thecompositions of the present invention can include Argania spinosa(argan) seed kernel extract, Secale cereale (rye) seed extract, Linumusitatissimum (linseed) seed extract, Malpighia punicifolia (acerola)fruit extract, Terminalia ferdinandiana (kakadu plum) fruit extract,Myrciaria dubia (camu camu) fruit extract, Punica granatum(pomegrannate) fruit extract or sterols, Pinus sylvestris bark extract,Ribes nigrum (black currant) leaf extract, palmitoyl oligopeptide,palmitoyl tetrapeptide-7, or adenosine, or any combination thereof.Further, as shown in the figures and examples (which are incorporatedinto this section by reference), the inventors have discovered that thecombination of anyone of Argania spinosa (argan) seed kernel extract,Secale cereale (rye) seed extract, Linum usitatissimum (linseed) seedextract, Malpighia punicifolia (acerola) fruit extract, Terminaliaferdinandiana (kakadu plum) fruit extract, Myrciaria dubia (camu camu)fruit extract, Punica granatum (pomegrannate) fruit extract or sterols,Pinus sylvestris bark extract, Ribes nigrum (black currant) leafextract, palmitoyl oligopeptide, palmitoyl tetrapeptide-7, or adenosineproduce synergistic and complimentary effects that are beneficial toskin.

In certain embodiments, the compositions are formulated into topicalskin care compositions. The compositions can be cosmetic compositions.In other aspects, the compositions can be included in a cosmeticvehicle. Non-limiting examples of cosmetic vehicles are disclosed inother sections of this specification and are known to those of skill inthe art. Examples of cosmetic vehicles include emulsions (e.g.,oil-in-water and water-in-oil emulsions), creams, lotions, solutions(e.g., aqueous or hydro-alcoholic solutions), anhydrous bases (e.g.,lipstick or a powder), gels, and ointments. In other non-limitingembodiments, the compositions of the present invention can be includedin anti-aging, cleansing, or moisturizing products. The compositions canalso be formulated for topical skin application at least 1, 2, 3, 4, 5,6, 7, or more times a day during use. In other aspects of the presentinvention, compositions can be storage stable or color stable, or both.It is also contemplated that the viscosity of the composition can beselected to achieve a desired result (e.g., depending on the type ofcomposition desired, the viscosity of such composition can be from about1 cps to well over 1 million cps or any range or integer derivabletherein (e.g., 2 cps, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70,80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 2000, 3000,4000, 5000, 6000, 7000, 8000, 9000, 10000, 20000, 30000, 40000, 50000,60000, 70000, 80000, 90000, 100000, 200000, 300000, 400000, 500000,600000, 700000, 800000, 900000, 1000000 cps, etc., as measured on aBrookfield Viscometer using a TC spindle at 2.5 rpm at 25° C.). Inparticular embodiments, the composition has a viscosity ranging from30,000 to 50,000 cps. The compositions in non-limiting aspects can havea pH of about 6 to about 9. In other aspects, the pH can be 1, 2, 3, 4,5, 6, 7, 8, 9, 10, 11, 12, 13, or 14. In other aspects, the compositionscan be sunscreens having a sun protection factor (SPF) of 1, 5, 10, 15,20, 25, 30, 35, 40, 45, 50, 55, or more.

In particular aspects, the compositions can be oil-free, substantiallyanhydrous, and/or anhydrous. Other aspects include compositions havingwater.

The compositions of the present invention can include from about 0.001%to about 50%, by weight, of Argania spinosa (argan) seed kernel extract,Secale cereale (rye) seed extract, Linum usitatissimum (linseed) seedextract, Malpighia punicifolia (acerola) fruit extract, Terminaliaferdinandiana (kakadu plum) fruit extract, Myrciaria dubia (camu camu)fruit extract, Punica granatum (pomegrannate) fruit extract or sterols,Pinus sylvestris bark extract, Ribes nigrum (black currant) leafextract, palmitoyl oligopeptide, palmitoyl tetrapeptide-7, or adenosine,or any combination thereof. It should be recognized, however, that theamount of such ingredients within a composition can be modified below,within, or above this range based on the desired results. Therefore, theamount of such ingredients can include less than 0.0001%. In otheraspects, the compositions can include 0.0001, 0.0002 . . . 0.002, 0.003,0.004 . . . 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17,18, 19, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 95, 96, 97, 98, 99%,or more or, or any range derivable therein, by weight or volume ofArgania spinosa (argan) seed kernel extract, Secale cereale (rye) seedextract, Linum usitatissimum (linseed) seed extract, Malpighiapunicifolia (acerola) fruit extract, Terminalia ferdinandiana (kakaduplum) fruit extract, Myrciaria dubia (camu camu) fruit extract, Punicagranatum (pomegrannate) fruit extract or sterols, Pinus sylvestris barkextract, Ribes nigrum (black currant) leaf extract, palmitoyloligopeptide, palmitoyl tetrapeptide-7, or adenosine, or any combinationthereof.

The compositions of the present invention can also be modified to have adesired oxygen radical absorbance capacity (ORAC) value. In certainnon-limiting aspects, the compositions of the present invention can bemodified to have an ORAC value per mg of at least about 1, 2, 3, 4, 5,6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24,25, 26, 27, 28, 29, 30, 35, 40, 45, 50, 55, 60, 70, 80, 90, 95, 100,200, 300, 400, 500, 600, 700, 800, 900, 1000, 2000, 3000, 4000, 5000,6000, 7000, 8000, 9000, 10000, 15000, 20000, 30000, 50000, 100000 ormore or any range derivable therein.

In other non-limiting aspects of the present invention, the compositionscan further include a vitamin, a mineral, an essential fatty acid, anamino acid, a flavonoid, and/or a protein, or a combination thereof.Non-limiting examples of vitamins include the B vitamins (e.g., B1, B2,B6, B12, niacin, folic acid, biotin, and pantothenic acid), vitamin C,vitamin D, vitamin E (e.g., tocopherol or tocopheryl acetate), vitamin A(e.g., palmitate, retinyl palmitate, or retinoic acid), and vitamin K.Non-limiting examples of minerals include iron, potassium, phosphorus,magnesium, manganese, selenium, and calcium. Non-limiting examples ofessential fatty acids include Omega 3 (linolenic acid), Omega 6(linoleic acid) and Omega 9 (oleic acid) essential fatty acid, or acombination thereof. Non-limiting examples of amino acids includeessential amino acids (e.g., lysine, leucine, isoleucine, methionine,phenylalanine, threonine, tryptophan, valine, histidine, or arginine)and non-essential amino acids (e.g., serine, asparagine, glutamine,aspartic acid, glutamic acid, alanine, tyrosine, cysteine, glycine, orproline). Non-limiting examples of flavonoids include anthocyanincompounds (e.g., cyanidin-3-glucoside and cyanidin-3-rutinoside).

The compositions can include a triglyceride, a preservative, anessential oil, a UV absorption ingredient, and/or additional ingredientsdescribed in the specification and known in the art, and any combinationthereof. Non-limiting examples of triglycerides include small, medium,and large chain triglycerides. Non-limiting examples of preservativesinclude methylparaben, propylparaben, or a mixture of methylparaben andpropylparaben. Non-limiting examples of essential oils are thosedescribed in the specification and those known to a person of ordinaryskill in the art. Examples include sesame oil, macadamia nut oil, teatree oil, evening primrose oil, Spanish sage oil, Spanish rosemary oil,Coriander oil, Thyme oil, or Pimento berries oil. Non limiting examplesof UV absorption ingredients include dibenzoylmethane derivatives (e.g.,avobenzone), octocrylene, oxybenzone, homosalate, octisalate, octylmethoxycinnamate, ecamsule, titanium dioxide, zinc oxide, etc., andothers described in the specification and known to those in the art, andany combination thereof.

In other aspects, the compositions of the present invention can includeany one of, any combination of, or all of the plant extracts disclosedthroughout this specification along with any other cosmetic orpharmaceutical ingredient described in this specification. For instance,the compositions can include any one of, any combination of, or all ofthe plant extracts in combination with any one of, any combination of,or all of the UV absorbing agents, moisturizing agents, antioxidants,structuring agents, emulsifiers, silicone containing compounds,essential oils, thickening agents, and/or preservatives disclosed inthis specification. The amounts of such ingredients can range from0.0001 to 99% or any range or integer derivable therein as disclosed inthis specification.

In particular aspects, there is disclosed a topical skin carecomposition comprising: (a) a combination of the following plantextracts: Malpighia punicifolia (acerola) extract; Argania spinosa(argan) extract; Myrciaria dubia (camu camu) extract; Punica granatum(pomegrannate) extract; Pinus sylvestris extract; Terminaliaferdinandiana (kakadu plum) extract; and Linum usitatissimum (linseed)extract; and (b) a dermatologically acceptable vehicle. Thedermatologically acceptable vehicle can include: water; glycerin;dimethicone or cyclomethicone; hydrogenated polydecene; butylene glycol;propylene glycol; and betaine. In certain aspects, the dermatologicallyacceptable vehicle comprises: at least 60% by weight of water; 3 to 10%by weight of glycerin; 2 to 10% by weight of dimethicone orcyclomethicone; 2 to 10% by weight of hydrogenated polydecene; 0.5 to 3%by weight of butylene glycol; 0.5 to 3% by weight of propylene glycol;and 0.5 to 3% by weight of betaine. In certain aspects, the compositionfurther includes adenosine. The composition can be formulated as aserum, cream, lotion, emulsion, etc. or any other vehicle disclosedthroughout this specification. In particular aspects, the compositionhas a viscosity ranging from 30,000 to 50,000 cps as measured by aBrookfield Viscometer using a TC spindle at 2.5 rpm at 25° C. and aoxygen radical absorbance capacity (ORAC) value per mg of at least about5.0 to about 25.0. The composition can also include the following plantextracts: algae extract; yeast extract; Secale cereale (rye) seedextract; and Ribes nigrum (black current) leaf extract. In moreparticular aspects, the topical skin care composition includes Malpighiapunicifolia (acerola) fruit extract; Argania spinosa (argan) seed kernelextract; Myrciaria dubia (camu camu) fruit extract; Punica granatum(pomegrannate) fruit extract or sterols; Pinus sylvestris bark extract;Terminalia ferdinandiana fruit (kakadu plum) extract; and Linumusitatissimum (linseed) seed extract. The amount of each plant extractcan range from 0.00001 to 99% or any range or integer derivable therein.In particular embodiments, the plant extracts can each be included inthe composition in a range from 0.001 to 3% by weight or volume of thecomposition.

In one embodiment, the topical skin care composition can be any one ofthe compositions disclosed in Tables 1-5 of this specification. TheTable 5 composition is a particular non-limiting formulation.

Also disclosed is a method of treating or preventing a skin conditioncomprising topical application of a composition comprising Arganiaspinosa (argan) seed kernel extract, Secale cereale (rye) seed extract,Linum usitatissimum (linseed) seed extract, Malpighia punicifolia(acerola) fruit extract, Terminalia ferdinandiana (kakadu plum) fruitextract, Myrciaria dubia (camu camu) fruit extract, Punica granatum(pomegrannate) fruit extract or sterols, Pinus sylvestris bark extract,Ribes nigrum (black currant) leaf extract, palmitoyl oligopeptide,palmitoyl tetrapeptide-7, or adenosine, or any combination thereof,wherein the topical application of the composition treats the skincondition. The method can include topical application of the compositionto a portion of skin in need of such composition (e.g., skin having askin condition), wherein topical application reduces or prevents theskin condition when compared to skin that has a skin condition and thathas not been treated with the composition. Non-limiting examples of skinconditions include pruritus, spider veins, lentigo, age spots, senilepurpura, keratosis, melasma, blotches, fine lines or wrinkles, nodules,sun damaged skin, dermatitis (including, but not limited to seborrheicdermatitis, nummular dermatitis, contact dermatitis, atopic dermatitis,exfoliative dermatitis, perioral dermatitis, and stasis dermatitis),psoriasis, folliculitis, rosacea, acne, impetigo, erysipelas,erythrasma, eczema, and other inflammatory skin conditions. In certainnon-limiting aspects, the skin condition can be caused by exposure to UVlight, age, irradiation, chronic sun exposure, environmental pollutants,air pollution, wind, cold, heat, chemicals, disease pathologies,smoking, or lack of nutrition. The skin can be facial skin or non-facialskin (e.g., arms, legs, hands, chest, back, feet, etc.). The method canfurther comprise identifying a person in need of skin treatment. Theperson can be a male or female. The age of the person can be at least 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65,70, 75, 80, 85, 90, 95, or more years old, or any range derivabletherein. The method can also include topically applying an amounteffective to: increase the stratum corneum turnover rate of the skin;increase collagen synthesis in fibroblasts; increase cellularanti-oxidant defense mechanisms (e.g., exogenous additions ofanti-oxidants can bolster, replenish, or prevent the loss of cellularantioxidants such as catalase and glutathione in skin cells (e.g.,keratinocytes, melanocytes, langerhans cells, etc.) which will reduce orprevent oxidative damage to the skin, cellular, proteins, and lipids);inhibit melanin production in melanocytes; reduce or prevent oxidativedamage to skin (including reducing the amount lipid peroxides and/orprotein oxidation in the skin).

In certain embodiments, compositions of the present invention candecrease the amount of internal oxidation and/or external oxidativedamage in a cell. In other aspects, the compositions can increasecollagen synthesis in a cell. The compositions can also reduce skininflammation, such as by reducing inflammatory cytokine production in acell. Non-limiting examples of such cells include human epidermalkeratinocyte, human fibroblast dermal cell, human melanocytes, threedimensional human cell-derived in vitro tissue equivalents comprisinghuman keratinocytes, human fibroblasts, or human melanocytes, or anycombination thereof (e.g., combination of human keratinocytes and humanfibroblasts or a combination of human keratinocytes and humanmelanocytes).

Also disclosed is a method of lightening skin or evening skin tonecomprising applying the compositions of the present invention to theskin. The method can further comprise identify a person in need oflightening skin or evening skin tone. The methods can further includeinhibiting melanogenesis in a skin cell, inhibiting tyrosinase ortyrosinase synthesis in a skin cell, or inhibiting melanin transport tokeratinocytes in a skin cell. The composition can act as an alphamelanin stimulatory hormone antagonist. The composition can even outpigmentation of the skin. In non-limiting aspect, lightening skin caninclude reducing the appearance of an age spot, a skin discoloration, ora freckle by topical application of the composition to skin having anage spot, skin discoloration, a freckle, etc.

Also disclosed is a method of treating hyperpigmentation comprisingapplying the compositions of the present invention to the skin. Themethod can also comprise identifying a person in need of treatinghyperpigmentation. Additional methods contemplated by the inventorinclude methods for reducing the appearance of an age spot, a skindiscoloration, or a freckle, reducing or preventing the appearance offine lines or wrinkles in skin, or increasing the firmness of skin.

In a particular embodiment there is disclosed a method of reducing theappearance of a skin condition comprising topically applying any one ofthe compositions described in this specification to the skin condition,wherein topical application of the composition to skin condition reducesthe appearance of the skin condition. The skin condition can be any oneof those described in this specification. In particular aspects, theskin condition is a fine line or wrinkle, uneven skin tone, or an agespot. The skin condition can be located on facial skin, arm skin, legskin, chest skin, abdomen skin, back skin etc.

In yet another embodiment there is disclosed a method of increasing thefirmness of skin comprising topically applying any one of thecompositions described in this specification to skin in need thereof (anon-limiting example of which can be sagging skin, aged skin, skin thathas reduced elasticity, skin that has skin cells having inadequateamounts of collage, fibronectin, or laminin or all of such proteins,etc.), wherein topical application of the composition to skin increasesthe firmness of skin. The composition can be used on facial skin, armskin, leg skin, chest skin, abdomen skin, back skin, etc.

In still another embodiment there is disclosed a method of increasingcollagen, fibronectin, or laminin production in a skin cell comprisingtopically applying any one of the compositions disclosed in thisspecification to a skin cell that is in need of collagen, fibronectin,or laminin production, wherein the topical application of thecomposition to the skin cell increases collagen, fibronectin, or lamininproduction in the skin cell. Typical skin cells can be found in skinthat is aged, damaged by UV radiation, damaged by environmental factors,wrinkles, etc. In certain aspects, collagen, fibronectin, and lamininproduction are increased in the skin cell.

Compositions comprising Argania spinosa (argan) seed kernel extract,Secale cereale (rye) seed extract, Linum usitatissimum (linseed) seedextract, Malpighia punicifolia (acerola) fruit extract, Terminaliaferdinandiana (kakadu plum) fruit extract, Myrciaria dubia (camu camu)fruit extract, Punica granatum (pomegrannate) fruit extract or sterols,Pinus sylvestris bark extract, Ribes nigrum (black currant) leafextract, palmitoyl oligopeptide, palmitoyl tetrapeptide-7, or adenosine,or any combination thereof can produce synergistic effects. For example,such ingredients can work together synergistically to produce effectsthat exceed the effects of what would be expected if the extracts wereused in separate compositions. Non-limiting synergistic effects includethe reduction of internal or external oxidative damage, increasedcollagen production, reduction in inflammatory responses and theinhibition of melanogenesis.

Compositions comprising Argania spinosa (argan) seed kernel extract,Secale cereale (rye) seed extract, Linum usitatissimum (linseed) seedextract, Malpighia punicifolia (acerola) fruit extract, Terminaliaferdinandiana (kakadu plum) fruit extract, Myrciaria dubia (camu camu)fruit extract, Punica granatum (pomegrannate) fruit extract or sterols,Pinus sylvestris bark extract, Ribes nigrum (black currant) leafextract, palmitoyl oligopeptide, palmitoyl tetrapeptide-7, or adenosine,or any combination thereof, can also act in a complementary fashion. Forexample, such ingredients can be used to reduce inflammatory responses(e.g., the reduction of inflammatory cytokine production) by certaincytokines that are not reduced, or not as significantly reduced, byother ingredients, and vice-versa.

Also contemplated are kits that includes the compositions of the presentinvention. In certain embodiments, the composition is comprised in acontainer. The container can be a bottle, dispenser, or package. Thecontainer can dispense a pre-determined amount of the composition. Incertain aspects, the compositions is dispensed in a spray, dollop, orliquid. The container can include indicia on its surface. The indiciacan be a word, an abbreviation, a picture, or a symbol.

Also contemplated is a product comprising a composition of the presentinvention. In non-limiting aspects, the product can be a cosmeticproduct. The cosmetic product can be those described in other sectionsof this specification or those known to a person of skill in the art.Non-limiting examples of products include a moisturizer, a cream, alotion, a skin softener, a foundation, a night cream, a lipstick, acleanser, a toner, a sunscreen, a mask, or an anti-aging product.

The compositions and methods for their use can “comprise,” “consistessentially of,” or “consist of” any of the ingredients disclosedthroughout the specification.

It is contemplated that any embodiment discussed in this specificationcan be implemented with respect to any method or composition of theinvention, and vice versa. Furthermore, compositions of the inventioncan be used to achieve methods of the invention.

In one embodiment, compositions of the present invention can bepharmaceutically or cosmetically elegant. “Pharmaceutically elegant”and/or “cosmetically elegant” describes a composition that hasparticular tactile properties which feel pleasant on the skin (e.g.,compositions that are not too watery or greasy, compositions that have asilky texture, compositions that are non-tacky or sticky, etc.).Pharmaceutically or cosmetically elegant can also relate to thecreaminess or lubricity properties of the composition or to the moistureretaining properties of the composition.

“Topical application” means to apply or spread a composition onto thesurface of keratinous tissue. “Topical skin composition” includescompositions suitable for topical application on keratinous tissue. Suchcompositions are typically dermatologically-acceptable in that they donot have undue toxicity, incompatibility, instability, allergicresponse, and the like, when applied to skin. Topical skin carecompositions of the present invention can have a selected viscosity toavoid significant dripping or pooling after application to skin.

“Dermatologically acceptable carrier, vehicle, or medium” means acarrier, vehicle, or medium into which the active ingredients can beeffectively incorporated into. A dermatologically acceptable carrier isdesign to reduce or avoid undue toxicity, incompatibility, instability,allergic response, and the like, when applied to skin.

“Keratinous tissue” includes keratin-containing layers disposed as theoutermost protective covering of mammals and includes, but is notlimited to, skin, hair and nails.

A “non-volatile oil” includes those substance that will not evaporate atordinary or room temperature.

The terms “mixture,” “mix,” and “mixing” or any variants of these terms,when used in the claims and/or specification includes, stirring,blending, dispersing, milling, homogenizing, and other similar methods.The mixing of the components or ingredients of the disclosedcompositions can form into a solution. In other embodiments, themixtures may not form a solution. The ingredients/components can alsoexist as undissolved colloidal suspensions.

The term “about” or “approximately” are defined as being close to asunderstood by one of ordinary skill in the art, and in one non-limitingembodiment the terms are defined to be within 10%, preferably within 5%,more preferably within 1%, and most preferably within 0.5%.

The term “substantially” and its variations are defined as being largelybut not necessarily wholly what is specified as understood by one ofordinary skill in the art, and in one non-limiting embodimentsubstantially refers to ranges within 10%, within 5%, within 1%, orwithin 0.5%.

The terms “treating,” “inhibiting,” or “reducing” or any variation ofthese terms, when used in the claims and/or the specification includesany measurable decrease or complete inhibition to achieve a desiredresult.

The term “effective,” as that term is used in the specification and/orclaims, means adequate to accomplish a desired, expected, or intendedresult.

The use of the word “a” or “an” when used in conjunction with the term“comprising” in the claims and/or the specification may mean “one,” butit is also consistent with the meaning of “one or more,” “at least one,”and “one or more than one.”

The use of the term “or” in the claims is used to mean “and/or” unlessexplicitly indicated to refer to alternatives only or the alternativesare mutually exclusive, although the disclosure supports a definitionthat refers to only alternatives and “and/or.”

As used in this specification and claim(s), the words “comprising” (andany form of comprising, such as “comprise” and “comprises”), “having”(and any form of having, such as “have” and “has”), “including” (and anyform of including, such as “includes” and “include”) or “containing”(and any form of containing, such as “contains” and “contain”) areinclusive or open-ended and do not exclude additional, unrecitedelements or method steps.

Other objects, features and advantages of the present invention willbecome apparent from the following detailed description. It should beunderstood, however, that the detailed description and the examples,while indicating specific embodiments of the invention, are given by wayof illustration only. Additionally, it is contemplated that changes andmodifications within the spirit and scope of the invention will becomeapparent to those skilled in the art from this detailed description.

BRIEF DESCRIPTION OF THE DRAWINGS

The following drawings form part of the present specification and areincluded to further demonstrate certain aspects of the presentinvention. The invention may be better understood by reference to one ormore of these drawings in combination with the detailed description ofspecific embodiments presented below.

FIG. 1. Plant-derived extracts stimulate matrix protein synthesis. Humandermal fibroblasts were treated with each extract (1.0% final conc.) inculture media for 3 days. Cell supernatants were collected and analyzedfor the presence of various matrix proteins by ELISA. Measurements werenormalized for cellular metabolic activity, as determined bybioconversion of MTS.

FIG. 2. Plant-derived extracts inhibit activity of MMP. Recombinant MMP2 (gelatinase A), 3 (stromelysin-1), or 9 (gelatinase B) was incubatedwith 1% solutions of each extract in the presence of a chromogenicsubstrate. Enzyme activity was measured as absorbance over time. Data isexpressed as % inhibition of enzyme activity: (−)=10-30% decrease;(−−)=30-50% decrease; (−−−)=50-100% decrease.

FIG. 3. Topical application of a combined set of extracts stimulatesmatrix protein synthesis in skin tissue equivalent. Simple cosmeticformulations, with or without plant extracts, were topically applied toskin equivalent tissues. Supernatants were collected following 3 days oftreatment and analyzed for the presence of various matrix proteins byELISA. Data are expressed as % change from formulation control (withoutplant extracts). (* indicates p<0.05 by unpaired t test.). The combinedset of extracts included Malpighia punicifolia (acerola) extract;Argania spinosa (argan) extract; Myrciaria dubia (camu camu) extract;Punica granatum (pomegrannate) extract; Pinus sylvestris extract;Terminalia ferdinandiana (kakadu plum) extract; Linum usitatissimum(linseed) extract; Ribes nigrum (black current) extract; Secale cereale(rye) extract; algae extract; and yeast extract.

FIG. 4. Application of a cosmetic formulation containing the combinedset of extracts from FIG. 3 improves facial attributes. A baselinecontrolled, clinical efficacy study was performed using independentpanelists with mild to moderate fine lines and wrinkles. Panelists wereevaluated by an expert grader for visual assessments of facialattributes. Overall visual grading improved over baseline at weeks 4 and7. Remaining attributes, fine wrinkle and coarse wrinkle parameters,showed significant improved at both weeks 4 and 7.

FIG. 5. Biomechanical Tissue Characterization of human skin followingapplication of the formulation from FIG. 5. A baseline controlled,clinical efficacy study performed using independent panelists with mildto moderate fine lines and wrinkles. Panelists were evaluated using theBiomechanical Tissue Characterization (BTC-2000) instrument to assessskin visio-elasticity. Measurements were taken in triplicate from thecanthus and cheek area. Pliability, softness and dryness continued toimprove at week 7.

DESCRIPTION OF ILLUSTRATIVE EMBODIMENTS

In today's image conscious society, people are continually looking for aproduct that can improve the visual appearance of their skin. Oftentimes, aged skin, uneven skin tone, or skin damaged by environmentalfactors such as UV light, chronic sun exposure, environmentalpollutants, chemicals, disease pathologies, or smoking, is associatedwith unattractive skin. Previous attempts to improve the visualappearance of skin has been shown to have various drawbacks such as skinirritation and prolonged recovery periods.

The present invention is an effective alternative to the use of retinoidcompounds or other compositions and ingredients currently used to treataged skin, environmentally-damaged skin, uneven skin tone, and otherskin conditions. In one non-limiting aspect, the present invention canbe used to improve the skin's visual appearance, physiologicalfunctions, clinical properties, or biophysical properties by providingtopical skin care formulations that include Argania spinosa (argan) seedkernel extract, Secale cereale (rye) seed extract, Linum usitatissimum(linseed) seed extract, Malpighia punicifolia (acerola) fruit extract,Terminalia ferdinandiana (kakadu plum) fruit extract, Myrciaria dubia(camu camu) fruit extract, Punica granatum (pomegrannate) fruit extractor sterols, Pinus sylvestris bark extract, Ribes nigrum (black currant)leaf extract, palmitoyl oligopeptide, palmitoyl tetrapeptide-7, oradenosine, or any combination thereof. These and other aspect of thepresent invention are described in further detail below.

A. Active Ingredients

As explained above, topical skin care compositions of the presentinvention can include Argania spinosa (argan) seed kernel extract,Secale cereale (rye) seed extract, Linum usitatissimum (linseed) seedextract, Malpighia punicifolia (acerola) fruit extract, Terminaliaferdinandiana (kakadu plum) fruit extract, Myrciaria dubia (camu camu)fruit extract, Punica granatum (pomegrannate) fruit extract or sterols,Pinus sylvestris bark extract, Ribes nigrum (black currant) leafextract, palmitoyl oligopeptide, palmitoyl tetrapeptide-7, or adenosine,or any combination thereof. These ingredients can be obtained from thirdparty sellers. For instance, Argania spinosa (argan) seed kernel extractcan be purchased from Laboratories Serobiologiques (France) under thetrade name ARGATENSYL LS™ or from Pentapharm/Centerchem (USA) under thetrade name REGU-SEB™. Secale cereale (rye) seed extract from Silab(France) under the trade names COHELISS™ or MX029-ADAPTINE™ or fromLaboratories Serbiologiques (France) under the trade name HSP-BALANCELS™. Linum usitatissimum (linseed) seed extract can be purchased fromLucas Meyer Cosmetics (France) under the trade name LINUMINE™ or fromLaboratories Serobiologiques (France) under the trade name ALMONDERMINLS™. Malpighia punicifolia (acerola) fruit extract can be purchased fromGreentech S.A. (France) under the trade names ACEROMINE™, ROSAMINE™, orROSAMINE II™. Terminalia ferdinandiana (kakadu plum) extract can bepurchased from Southern Cross Botanicals (Australia). Myrciaria dubia(camu camu) fruit extract can be purchased from Amax NutraSource (USA)under the trade name CAMU CAMU EXTRACT™ and from Nichirei (Japan) underthe trade names CAMU-CAMU EXTRACT B30™ or CAMU-CAMU EXTRACT W™. Punicagranatum (pomegrannate) fruit extract can be purchased from ActiveOrganics (USA) under the trade names CO ACTIPHYTE OF POMEGRANATE AJ™, COACTIPHYTE OF POMEGRANATE GL™, CO ACTIPHYTE OF POMEGRANATE LIPO O™, COACTIPHYTE OF POMEGRANATE LIPO RS™, CO ACTIPHYTE OF POMEGRANATE LIPO S™,and CO ACTIPHYTE OF POMEGRANATE LIPO SUN™ Punica granatum (pomegrannate)sterols, sterols obtained from pomegrannate fruit and/or seeds, can bepurchased from Active Concepts (USA) under the trade name ABSPOMEGRANATE STEROLS™. Pinus sylvestris bark extract can be purchasedfrom Greentech S.A. (France) under the trade name EPICA™. Ribes nigrum(black currant) leaf extract can be purchased from Greentech S.A.(France) under the trade name EPICA™ and from Alban Muller International(France) under the trade names PHYTAMI of ORGANIC BLACKCURRANT™ andBLACKCURRANT LEAF HS™. Palmitoyl oligopeptide, the palmitic acid esterof a synthetic peptide of at least two amino acids selected fromalanine, arginine, aspartic acid, glycine, histadine, lysine, proline,serine, or valine, can be purchased from Sederma (France) under thetrade names BIO-BUSTYL™, BIOPEPTIDE-CL™, BIOPEPTIDE-EL™, DERMAXYL™,Haloxyl™, MATRIXYL 3000™, and MAXI-LIP™. Palmitoyl tetrapeptide-7, thereaction product of palmitic acid and tetrapepetide-7 (Gly-Gln-Pro-Arg),can be purchased from Sederma (France) under the trade names EYELISS™,HALOXYL™, MATRIXYL 3000™, and RIGIN™. Adenosine conforms to thefollowing formula and can be purchased from a wide variety of suppliers,including Naturogin Inc. (Korea) and Biospectrum (India):

Additional information and suppliers of the above-listed ingredients(and the corresponding trade names) can be found in InternationalCosmetic Ingredient Dictionary Handbook, 12^(th) Edition (2008), whichis incorporated by reference. Further, the extracts identified above canbe produced by obtaining the corresponding fruit, seed, or leaf, toproduce the extract by extraction methods which are known to those ofordinary skill in the art. The inventors also contemplate that otherportions of the substrate (e.g., argania spinosa (argan), Secale cereale(rye), Linum usitatissimum (linseed), Malpighia punicifolia (acerola),Terminalia ferdinandiana (kakadu plum), Myrciaria dubia (camu camu),Punica granatum (pomegrannate), Pinus sylvestris (pinus), Ribes nigrum(black currant)) producing the extract can be used in the compositionsand methods of the present invention. Non-limiting examples of the otherportions include the whole fruit, whole vegetable, whole plant, wholetree, whole bush, seed, peel, fruit, stem, bark, leaf, root, flower,petal, bulb, etc. These other portions are described in theInternational Cosmetic Ingredient Dictionary Handbook, 12^(th) Edition(2008), which is incorporated by reference.

B. Oxygen Radical Absorbance Capacity

Oxygen Radical Absorption (or Absorbance) Capacity (ORAC) is an assaythat measures the antioxidant activity of an ingredient or composition.In essence, it can quantify the degree and length of time it takes toinhibit the action of an oxidizing agent such as oxygen radicals thatare known to cause damage cells (e.g., skin cells). The ORAC value ofthe compositions of the present invention can be determined by methodsknown to those of ordinary skill in the art (see U.S. Publication Nos.2004/0109905 and 2005/0163880; Cao et al. (1993)), all of which areincorporated by reference). In summary, the assay described in Cao etal. (1993) measures the ability of antioxidant compounds in testmaterials to inhibit the decline of B-phycoerythrm (B-PE) fluorescencethat is induced by a peroxyl radical generator, AAPH.

C. Compositions of the Present Invention

It is contemplated that the compositions of the present invention caninclude Argania spinosa (argan) seed kernel extract, Secale cereale(rye) seed extract, Linum usitatissimum (linseed) seed extract,Malpighia punicifolia (acerola) fruit extract, Terminalia ferdinandiana(kakadu plum) fruit extract, Myrciaria dubia (camu camu) fruit extract,Punica granatum (pomegrannate) fruit extract or sterols, Pinussylvestris bark extract, Ribes nigrum (black currant) leaf extract,palmitoyl oligopeptide, palmitoyl tetrapeptide-7, or adenosine, or anycombination thereof. Additionally, the compositions can include anynumber of combinations of additional ingredients described throughoutthis specification. The concentrations of the any ingredient within thecompositions can vary. In non-limiting embodiments, for example, thecompositions can comprise, consisting essentially of, or consist of, intheir final form, for example, at least about 0.0001%, 0.0002%, 0.0003%,0.0004%, 0.0005%, 0.0006%, 0.0007%, 0.0008%, 0.0009%, 0.0010%, 0.0011%,0.0012%, 0.0013%, 0.0014%, 0.0015%, 0.0016%, 0.0017%, 0.0018%, 0.0019%,0.0020%, 0.0021%, 0.0022%, 0.0023%, 0.0024%, 0.0025%, 0.0026%, 0.0027%,0.0028%, 0.0029%, 0.0030%, 0.0031%, 0.0032%, 0.0033%, 0.0034%, 0.0035%,0.0036%, 0.0037%, 0.0038%, 0.0039%, 0.0040%, 0.0041%, 0.0042%, 0.0043%,0.0044%, 0.0045%, 0.0046%, 0.0047%, 0.0048%, 0.0049%, 0.0050%, 0.0051%,0.0052%, 0.0053%, 0.0054%, 0.0055%, 0.0056%, 0.0057%, 0.0058%, 0.0059%,0.0060%, 0.0061%, 0.0062%, 0.0063%, 0.0064%, 0.0065%, 0.0066%, 0.0067%,0.0068%, 0.0069%, 0.0070%, 0.0071%, 0.0072%, 0.0073%, 0.0074%, 0.0075%,0.0076%, 0.0077%, 0.0078%, 0.0079%, 0.0080%, 0.0081%, 0.0082%, 0.0083%,0.0084%, 0.0085%, 0.0086%, 0.0087%, 0.0088%, 0.0089%, 0.0090%, 0.0091%,0.0092%, 0.0093%, 0.0094%, 0.0095%, 0.0096%, 0.0097%, 0.0098%, 0.0099%,0.0100%, 0.0200%, 0.0250%, 0.0275%, 0.0300%, 0.0325%, 0.0350%, 0.0375%,0.0400%, 0.0425%, 0.0450%, 0.0475%, 0.0500%, 0.0525%, 0.0550%, 0.0575%,0.0600%, 0.0625%, 0.0650%, 0.0675%, 0.0700%, 0.0725%, 0.0750%, 0.0775%,0.0800%, 0.0825%, 0.0850%, 0.0875%, 0.0900%, 0.0925%, 0.0950%, 0.0975%,0.1000%, 0.1250%, 0.1500%, 0.1750%, 0.2000%, 0.2250%, 0.2500%, 0.2750%,0.3000%, 0.3250%, 0.3500%, 0.3750%, 0.4000%, 0.4250%, 0.4500%, 0.4750%,0.5000%, 0.5250%, 0.0550%, 0.5750%, 0.6000%, 0.6250%, 0.6500%, 0.6750%,0.7000%, 0.7250%, 0.7500%, 0.7750%, 0.8000%, 0.8250%, 0.8500%, 0.8750%,0.9000%, 0.9250%, 0.9500%, 0.9750%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%,1.6%, 1.7%, 1.8%, 1.9%, 2.0%, 2.1%, 2.2%, 2.3%, 2.4%, 2.5%, 2.6%, 2.7%,2.8%, 2.9%, 3.0%, 3.1%, 3.2%, 3.3%, 3.4%, 3.5%, 3.6%, 3.7%, 3.8%, 3.9%,4.0%, 4.1%, 4.2%, 4.3%, 4.4%, 4.5%, 4.6%, 4.7%, 4.8%, 4.9%, 5.0%, 5.1%,5.2%, 5.3%, 5.4%, 5.5%, 5.6%, 5.7%, 5.8%, 5.9%, 6.0%, 6.1%, 6.2%, 6.3%,6.4%, 6.5%, 6.6%, 6.7%, 6.8%, 6.9%, 7.0%, 7.1%, 7.2%, 7.3%, 7.4%, 7.5%,7.6%, 7.7%, 7.8%, 7.9%, 8.0%, 8.1%, 8.2%, 8.3%, 8.4%, 8.5%, 8.6%, 8.7%,8.8%, 8.9%, 9.0%, 9.1%, 9.2%, 9.3%, 9.4%, 9.5%, 9.6%, 9.7%, 9.8%, 9.9%,10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%,24%, 25%, 26%, 27%, 28%, 29%, 30%, 35%, 40%, 45%, 50%, 60%, 65%, 70%,75%, 80%, 85%, 90%, 95%, or 99% or any range derivable therein, of atleast one of the ingredients that are mentioned throughout thespecification and claims. In non-limiting aspects, the percentage can becalculated by weight or volume of the total composition. A person ofordinary skill in the art would understand that the concentrations canvary depending on the addition, substitution, and/or subtraction ofingredients in a given composition.

The disclosed compositions of the present invention may also includevarious antioxidants to retard oxidation of one or more components.Additionally, the prevention of the action of microorganisms can bebrought about by preservatives such as various antibacterial andantifungal agents, including but not limited to parabens (e.g.,methylparabens, propylparabens), chlorobutanol, phenol, sorbic acid,thimerosal or combinations thereof.

D. Vehicles

The compositions of the present invention can be incorporated into alltypes of vehicles. Non-limiting examples of suitable vehicles includeemulsions (e.g., water-in-oil, water-in-oil-in-water, oil-in-water,oil-in-water-in-oil, oil-in-water-in-silicone emulsions), creams,lotions, solutions (both aqueous and hydro-alcoholic), anhydrous bases(such as lipsticks and powders), gels, and ointments or by other methodor any combination of the forgoing as would be known to one of ordinaryskill in the art (Remington's, 1990). Variations and other appropriatevehicles will be apparent to the skilled artisan and are appropriate foruse in the present invention. In certain aspects, it is important thatthe concentrations and combinations of the compounds, ingredients, andagents be selected in such a way that the combinations are chemicallycompatible and do not form complexes which precipitate from the finishedproduct.

It is also contemplated that ingredients identified throughout thisspecification, including but not limited to Argania spinosa (argan) seedkernel extract, Secale cereale (rye) seed extract, Linum usitatissimum(linseed) seed extract, Malpighia punicifolia (acerola) fruit extract,Terminalia ferdinandiana (kakadu plum) fruit extract, Myrciaria dubia(camu camu) fruit extract, Punica granatum (pomegrannate) fruit extractor sterols, Pinus sylvestris bark extract, Ribes nigrum (black currant)leaf extract, palmitoyl oligopeptide, palmitoyl tetrapeptide-7, oradenosine, or any combination thereof, can be individually orcombinatorially encapsulated for delivery to a target area such as skin.Non-limiting examples of encapsulation techniques include the use ofliposomes, vesicles, and/or nanoparticles (e.g., biodegradable andnon-biodegradable colloidal particles comprising polymeric materials inwhich the ingredient is trapped, encapsulated, and/or absorbed—examplesinclude nanospheres and nanocapsules) that can be used as deliveryvehicles to deliver the ingredient to skin (see, e.g., U.S. Pat. No.6,387,398; U.S. Pat. No. 6,203,802; U.S. Pat. No. 5,411,744; Kreuter1998).

E. Cosmetic Products and Articles of Manufacture

The composition of the present invention can also be used in manycosmetic products including, but not limited to, sunscreen products,sunless skin tanning products, hair products, finger nail products,moisturizing creams, skin benefit creams and lotions, softeners, daylotions, gels, ointments, foundations, night creams, lipsticks,cleansers, toners, masks, or other known cosmetic products orapplications. Additionally, the cosmetic products can be formulated asleave-on or rinse-off products. In certain aspects, the compositions ofthe present invention are stand-alone products.

F. Additional Ingredients

In addition to the Argania spinosa (argan) seed kernel extract, Secalecereale (rye) seed extract, Linum usitatissimum (linseed) seed extract,Malpighia punicifolia (acerola) fruit extract, Terminalia ferdinandiana(kakadu plum) fruit extract, Myrciaria dubia (camu camu) fruit extract,Punica granatum (pomegrannate) fruit extract or sterols, Pinussylvestris bark extract, Ribes nigrum (black currant) leaf extract,palmitoyl oligopeptide, palmitoyl tetrapeptide-7, and/or adenosineingredients disclosed throughout this specification, compositions of thepresent invention can include additional ingredients such as cosmeticingredients and pharmaceutical active ingredients. Non-limiting examplesof these additional ingredients are described in the followingsubsections.

1. Cosmetic Ingredients

The CTFA International Cosmetic Ingredient Dictionary and Handbook (2004and 2008) describes a wide variety of non-limiting cosmetic ingredientsthat can be used in the context of the present invention. Examples ofthese ingredient classes include: fragrances (artificial and natural),dyes and color ingredients (e.g., Blue 1, Blue 1 Lake, Red 40, titaniumdioxide, D&C blue no. 4, D&C green no. 5, D&C orange no. 4, D&C red no.17, D&C red no. 33, D&C violet no. 2, D&C yellow no. 10, and D&C yellowno. 11), adsorbents, lubricants, solvents, moisturizers (including,e.g., emollients, humectants, film formers, occlusive agents, and agentsthat affect the natural moisturization mechanisms of the skin),water-repellants, UV absorbers (physical and chemical absorbers such asparaminobenzoic acid (“PABA”) and corresponding PABA derivatives,titanium dioxide, zinc oxide, etc.), essential oils, vitamins (e.g. A,B, C, D, E, and K), trace metals (e.g. zinc, calcium and selenium),anti-irritants (e.g. steroids and non-steroidal anti-inflammatories),botanical extracts (e.g. aloe vera, chamomile, cucumber extract, ginkgobiloba, ginseng, and rosemary), anti-microbial agents, antioxidants(e.g., BHT and tocopherol), chelating agents (e.g., disodium EDTA andtetrasodium EDTA), preservatives (e.g., methylparaben andpropylparaben), pH adjusters (e.g., sodium hydroxide and citric acid),absorbents (e.g., aluminum starch octenylsuccinate, kaolin, corn starch,oat starch, cyclodextrin, talc, and zeolite), skin bleaching andlightening agents (e.g., hydroquinone and niacinamide lactate),humectants (e.g., sorbitol, urea, and manitol), exfoliants,waterproofing agents (e.g., magnesium/aluminum hydroxide stearate), skinconditioning agents (e.g., aloe extracts, allantoin, bisabolol,ceramides, dimethicone, hyaluronic acid, and dipotassium glycyrrhizate).Non-limiting examples of some of these ingredients are provided in thefollowing subsections.

a. UV Absorption Agents

UV absorption agents that can be used in combination with thecompositions of the present invention include chemical and physicalsunblocks. Non-limiting examples of chemical sunblocks that can be usedinclude para-aminobenzoic acid (PABA), PABA esters (glyceryl PABA,amyldimethyl PABA and octyldimethyl PABA), butyl PABA, ethyl PABA, ethyldihydroxypropyl PABA, benzophenones (oxybenzone, sulisobenzone,benzophenone, and benzophenone-1 through 12), cinnamates (octylmethoxycinnamate, isoamyl p-methoxycinnamate, octylmethoxy cinnamate,cinoxate, diisopropyl methyl cinnamate, DEA-methoxycinnamate, ethyldiisopropylcinnamate, glyceryl octanoate dimethoxycinnamate and ethylmethoxycinnamate), cinnamate esters, salicylates (homomethyl salicylate,benzyl salicylate, glycol salicylate, isopropylbenzyl salicylate, etc.),anthranilates, ethyl urocanate, homosalate, octisalate, dibenzoylmethanederivatives (e.g., avobenzone), octocrylene, octyl triazone, digalloytrioleate, glyceryl aminobenzoate, lawsone with dihydroxyacetone,ethylhexyl triazone, dioctyl butamido triazone, benzylidene malonatepolysiloxane, terephthalylidene dicamphor sulfonic acid, disodium phenyldibenzimidazole tetrasulfonate, diethylamino hydroxybenzoyl hexylbenzoate, bis diethylamino hydroxybenzoyl benzoate, bisbenzoxazoylphenyl ethylhexylimino triazine, drometrizole trisiloxane,methylene bis-benzotriazolyl tetramethylbutylphenol, andbis-ethylhexyloxyphenol methoxyphenyltriazine,4-methylbenzylidenecamphor, and isopentyl 4-methoxycinnamate.Non-limiting examples of physical sunblocks include, kaolin, talc,petrolatum and metal oxides (e.g., titanium dioxide and zinc oxide).Compositions of the present invention can have UVA and UVB absorptionproperties. The compositions can have an sun protection factor (SPF) of2, 3, 4, 56, 7, 8, 9, 10, 11, 12, 13, 14, 15, 20, 25, 30, 35, 40, 45,50, 55, 60, 70, 80, 90 or more, or any integer or derivative therein.

b. Moisturizing Agents

Non-limiting examples of moisturizing agents that can be used with thecompositions of the present invention include amino acids, chondroitinsulfate, diglycerin, erythritol, fructose, glucose, glycerin, glycerolpolymers, glycol, 1,2,6-hexanetriol, honey, hyaluronic acid,hydrogenated honey, hydrogenated starch hydrolysate, inositol, lactitol,maltitol, maltose, mannitol, natural moisturizing factor, PEG-15butanediol, polyglyceryl sorbitol, salts of pyrollidone carboxylic acid,potassium PCA, propylene glycol, sodium glucuronate, sodium PCA,sorbitol, sucrose, trehalose, urea, and xylitol.

Other examples include acetylated lanolin, acetylated lanolin alcohol,alanine, algae extract, aloe barbadensis, aloe-barbadensis extract, aloebarbadensis gel, althea officinalis extract, apricot (prunus armeniaca)kernel oil, arginine, arginine aspartate, arnica montana extract,aspartic acid, avocado (persea gratissima) oil, barrier sphingolipids,butyl alcohol, beeswax, behenyl alcohol, beta-sitosterol, birch (betulaalba) bark extract, borage (borago officinalis) extract, butcherbroom(ruscus aculeatus) extract, butylene glycol, calendula officinalisextract, calendula officinalis oil, candelilla (euphorbia cerifera) wax,canola oil, caprylic/capric triglyceride, cardamon (elettariacardamomum) oil, carnauba (copernicia cerifera) wax, carrot (daucuscarota sativa) oil, castor (ricinus communis) oil, ceramides, ceresin,ceteareth-5, ceteareth-12, ceteareth-20, cetearyl octanoate, ceteth-20,ceteth-24, cetyl acetate, cetyl octanoate, cetyl palmitate, chamomile(anthemis nobilis) oil, cholesterol, cholesterol esters, cholesterylhydroxystearate, citric acid, clary (salvia sclarea) oil, cocoa(theobroma cacao) butter, coco-caprylate/caprate, coconut (cocosnucifera) oil, collagen, collagen amino acids, corn (zea mays) oil,fatty acids, decyl oleate, dimethicone copolyol, dimethiconol, dioctyladipate, dioctyl succinate, dipentaerythrityl hexacaprylate/hexacaprate,DNA, erythritol, ethoxydiglycol, ethyl linoleate, eucalyptus globulusoil, evening primrose (oenothera biennis) oil, fatty acids, geraniummaculatum oil, glucosamine, glucose glutamate, glutamic acid,glycereth-26, glycerin, glycerol, glyceryl distearate, glycerylhydroxystearate, glyceryl laurate, glyceryl linoleate, glycerylmyristate, glyceryl oleate, glyceryl stearate, glyceryl stearate SE,glycine, glycol stearate, glycol stearate SE, glycosaminoglycans, grape(vitis vinifera) seed oil, hazel (corylus americana) nut oil, hazel(corylus avellana) nut oil, hexylene glycol, hyaluronic acid, hybridsafflower (carthamus tinctorius) oil, hydrogenated castor oil,hydrogenated coco-glycerides, hydrogenated coconut oil, hydrogenatedlanolin, hydrogenated lecithin, hydrogenated palm glyceride,hydrogenated palm kernel oil, hydrogenated soybean oil, hydrogenatedtallow glyceride, hydrogenated vegetable oil, hydrolyzed collagen,hydrolyzed elastin, hydrolyzed glycosaminoglycans, hydrolyzed keratin,hydrolyzed soy protein, hydroxylated lanolin, hydroxyproline, isocetylstearate, isocetyl stearoyl stearate, isodecyl oleate, isopropylisostearate, isopropyl lanolate, isopropyl myristate, isopropylpalmitate, isopropyl stearate, isostearamide DEA, isostearic acid,isostearyl lactate, isostearyl neopentanoate, jasmine (jasminumofficinale) oil, jojoba (buxus chinensis) oil, kelp, kukui (aleuritesmoluccana) nut oil, lactamide MEA, laneth-16, laneth-10 acetate,lanolin, lanolin acid, lanolin alcohol, lanolin oil, lanolin wax,lavender (lavandula angustifolia) oil, lecithin, lemon (citrus medicalimonum) oil, linoleic acid, linolenic acid, macadamia ternifolia nutoil, maltitol, matricaria (chamomilla recutita) oil, methyl glucosesesquistearate, methylsilanol PCA, mineral oil, mink oil, mortierellaoil, myristyl lactate, myristyl myristate, myristyl propionate,neopentyl glycol dicaprylate/dicaprate, octyldodecanol, octyldodecylmyristate, octyldodecyl stearoyl stearate, octyl hydroxystearate, octylpalmitate, octyl salicylate, octyl stearate, oleic acid, olive (oleaeuropaea) oil, orange (citrus aurantium dulcis) oil, palm (elaeisguineensis) oil, palmitic acid, pantethine, panthenol, panthenyl ethylether, paraffin, PCA, peach (prunus persica) kernel oil, peanut (arachishypogaea) oil, PEG-8 C12-18 ester, PEG-15 cocamine, PEG-150 distearate,PEG-60 glyceryl isostearate, PEG-5 glyceryl stearate, PEG-30 glycerylstearate, PEG-7 hydrogenated castor oil, PEG-40 hydrogenated castor oil,PEG-60 hydrogenated castor oil, PEG-20 methyl glucose sesquistearate,PEG40 sorbitan peroleate, PEG-5 soy sterol, PEG-10 soy sterol, PEG-2stearate, PEG-8 stearate, PEG-20 stearate, PEG-32 stearate, PEG40stearate, PEG-50 stearate, PEG-100 stearate, PEG-150 stearate,pentadecalactone, peppermint (mentha piperita) oil, petrolatum,phospholipids, polyamino sugar condensate, polyglyceryl-3 diisostearate,polyquaternium-24, polysorbate 20, polysorbate 40, polysorbate 60,polysorbate 80, polysorbate 85, potassium myristate, potassiumpalmitate, propylene glycol, propylene glycol dicaprylate/dicaprate,propylene glycol dioctanoate, propylene glycol dipelargonate, propyleneglycol laurate, propylene glycol stearate, propylene glycol stearate SE,PVP, pyridoxine dipalmitate, retinol, retinyl palmitate, rice (oryzasativa) bran oil, RNA, rosemary (rosmarinus officinalis) oil, rose oil,safflower (carthamus tinctorius) oil, sage (salvia officinalis) oil,sandalwood (santalum album) oil, serine, serum protein, sesame (sesamumindicum) oil, shea butter (butyrospermum parkii), silk powder, sodiumchondroitin sulfate, sodium hyaluronate, sodium lactate, sodiumpalmitate, sodium PCA, sodium polyglutamate, soluble collagen, sorbitanlaurate, sorbitan oleate, sorbitan palmitate, sorbitan sesquioleate,sorbitan stearate, sorbitol, soybean (glycine soja) oil, sphingolipids,squalane, squalene, stearamide MEA-stearate, stearic acid, stearoxydimethicone, stearoxytrimethylsilane, stearyl alcohol, stearylglycyrrhetinate, stearyl heptanoate, stearyl stearate, sunflower(helianthus annuus) seed oil, sweet almond (prunus amygdalus dulcis)oil, synthetic beeswax, tocopherol, tocopheryl acetate, tocopheryllinoleate, tribehenin, tridecyl neopentanoate, tridecyl stearate,triethanolamine, tristearin, urea, vegetable oil, water, waxes, wheat(triticum vulgare) germ oil, and ylang ylang (cananga odorata) oil.

c. Antioxidants

Non-limiting examples of antioxidants that can be used with thecompositions of the present invention include acetyl cysteine, ascorbicacid polypeptide, ascorbyl dipalmitate, ascorbyl methylsilanolpectinate, ascorbyl palmitate, ascorbyl stearate, BHA, BHT, t-butylhydroquinone, cysteine, cysteine HCl, diamylhydroquinone,di-t-butylhydroquinone, dicetyl thiodipropionate, dioleyl tocopherylmethylsilanol, disodium ascorbyl sulfate, distearyl thiodipropionate,ditridecyl thiodipropionate, dodecyl gallate, erythorbic acid, esters ofascorbic acid, ethyl ferulate, ferulic acid, gallic acid esters,hydroquinone, isooctyl thioglycolate, kojic acid, magnesium ascorbate,magnesium ascorbyl phosphate, methylsilanol ascorbate, natural botanicalanti-oxidants such as green tea or grape seed extracts,nordihydroguaiaretic acid, octyl gallate, phenylthioglycolic acid,potassium ascorbyl tocopheryl phosphate, potassium sulfite, propylgallate, quinones, rosmarinic acid, sodium ascorbate, sodium bisulfite,sodium erythorbate, sodium metabisulfite, sodium sulfite, superoxidedismutase, sodium thioglycolate, sorbityl furfural, thiodiglycol,thiodiglycolamide, thiodiglycolic acid, thioglycolic acid, thiolacticacid, thiosalicylic acid, tocophereth-5, tocophereth-10, tocophereth-12,tocophereth-18, tocophereth-50, tocopherol, tocophersolan, tocopherylacetate, tocopheryl linoleate, tocopheryl nicotinate, tocopherylsuccinate, and tris(nonylphenyl)phosphite.

d. Structuring Agents

In other non-limiting aspects, the compositions of the present inventioncan include a structuring agent. Structuring agent, in certain aspects,assist in providing rheological characteristics to the composition tocontribute to the composition's stability. In other aspects, structuringagents can also function as an emulsifier or surfactant. Non-limitingexamples of structuring agents include stearic acid, palmitic acid,stearyl alcohol, cetyl alcohol, behenyl alcohol, stearic acid, palmiticacid, the polyethylene glycol ether of stearyl alcohol having an averageof about 1 to about 21 ethylene oxide units, the polyethylene glycolether of cetyl alcohol having an average of about 1 to about 5 ethyleneoxide units, and mixtures thereof.

e. Emulsifiers

In certain aspects of the present invention, the compositions do notinclude an emulsifier. In other aspects, however, the compositions caninclude one or more emulsifiers. Emulsifiers can reduce the interfacialtension between phases and improve the formulation and stability of anemulsion. The emulsifiers can be nonionic, cationic, anionic, andzwitterionic emulsifiers (See McCutcheon's (1986); U.S. Pat. Nos.5,011,681; 4,421,769; 3,755,560). Non-limiting examples include estersof glycerin, esters of propylene glycol, fatty acid esters ofpolyethylene glycol, fatty acid esters of polypropylene glycol, estersof sorbitol, esters of sorbitan anhydrides, carboxylic acid copolymers,esters and ethers of glucose, ethoxylated ethers, ethoxylated alcohols,alkyl phosphates, polyoxyethylene fatty ether phosphates, fatty acidamides, acyl lactylates, soaps, TEA stearate, DEA oleth-3 phosphate,polyethylene glycol 20 sorbitan monolaurate (polysorbate 20),polyethylene glycol 5 soya sterol, steareth-2, steareth-20, steareth-21,ceteareth-20, PPG-2 methyl glucose ether distearate, ceteth-10,polysorbate 80, cetyl phosphate, potassium cetyl phosphate,diethanolamine cetyl phosphate, polysorbate 60, glyceryl stearate,PEG-100 stearate, and mixtures thereof.

f. Silicone Containing Compounds

In non-limiting aspects, silicone containing compounds include anymember of a family of polymeric products whose molecular backbone ismade up of alternating silicon and oxygen atoms with side groupsattached to the silicon atoms. By varying the —Si—O— chain lengths, sidegroups, and crosslinking, silicones can be synthesized into a widevariety of materials. They can vary in consistency from liquid to gel tosolids.

The silicone containing compounds that can be used in the context of thepresent invention include those described in this specification or thoseknown to a person of ordinary skill in the art. Non-limiting examplesinclude silicone oils (e.g., volatile and non-volatile oils), gels, andsolids. In certain aspects, the silicon containing compounds includes asilicone oils such as a polyorganosiloxane. Non-limiting examples ofpolyorganosiloxanes include dimethicone, cyclomethicone,polysilicone-11, phenyl trimethicone, trimethylsilylamodimethicone,stearoxytrimethylsilane, or mixtures of these and other organosiloxanematerials in any given ratio in order to achieve the desired consistencyand application characteristics depending upon the intended application(e.g., to a particular area such as the skin, hair, or eyes). A“volatile silicone oil” includes a silicone oil have a low heat ofvaporization, i.e. normally less than about 50 cal per gram of siliconeoil. Non-limiting examples of volatile silicone oils include:cyclomethicones such as Dow Corning 344 Fluid, Dow Corning 345 Fluid,Dow Corning 244 Fluid, and Dow Corning 245 Fluid, Volatile Silicon 7207(Union Carbide Corp., Danbury, Conn.); low viscosity dimethicones, i.e.dimethicones having a viscosity of about 50 cst or less (e.g.,dimethicones such as Dow Corning 200-0.5 cst Fluid). The Dow CorningFluids are available from Dow Corning Corporation, Midland, Mich.Cyclomethicone and dimethicone are described in the Third Edition of theCTFA Cosmetic Ingredient Dictionary (incorporated by reference) ascyclic dimethyl polysiloxane compounds and a mixture of fully methylatedlinear siloxane polymers end-blocked with trimethylsiloxy units,respectively. Other non-limiting volatile silicone oils that can be usedin the context of the present invention include those available fromGeneral Electric Co., Silicone Products Div., Waterford, N.Y. and SWSSilicones Div. of Stauffer Chemical Co., Adrian, Mich.

g. Essential Oils

Essential oils include oils derived from herbs, flowers, trees, andother plants. Such oils are typically present as tiny droplets betweenthe plant's cells, and can be extracted by several method known to thoseof skill in the art (e.g., steam distilled, enfleurage (i.e., extractionby using fat), maceration, solvent extraction, or mechanical pressing).When these types of oils are exposed to air they tend to evaporate(i.e., a volatile oil). As a result, many essential oils are colorless,but with age they can oxidize and become darker. Essential oils areinsoluble in water and are soluble in alcohol, ether, fixed oils(vegetal), and other organic solvents. Typical physical characteristicsfound in essential oils include boiling points that vary from about 160°to 240° C. and densities ranging from about 0.759 to about 1.096.

Essential oils typically are named by the plant from which the oil isfound. For example, rose oil or peppermint oil are derived from rose orpeppermint plants, respectively. Non-limiting examples of essential oilsthat can be used in the context of the present invention include sesameoil, macadamia nut oil, tea tree oil, evening primrose oil, Spanish sageoil, Spanish rosemary oil, coriander oil, thyme oil, pimento berriesoil, rose oil, anise oil, balsam oil, bergamot oil, rosewood oil, cedaroil, chamomile oil, sage oil, clary sage oil, clove oil, cypress oil,eucalyptus oil, fennel oil, sea fennel oil, frankincense oil, geraniumoil, ginger oil, grapefruit oil, jasmine oil, juniper oil, lavender oil,lemon oil, lemongrass oil, lime oil, mandarin oil, marjoram oil, myrrhoil, neroli oil, orange oil, patchouli oil, pepper oil, black pepperoil, petitgrain oil, pine oil, rose otto oil, rosemary oil, sandalwoodoil, spearmint oil, spikenard oil, vetiver oil, wintergreen oil, orylang ylang. Other essential oils known to those of skill in the art arealso contemplated as being useful within the context of the presentinvention.

h. Thickening Agents

Thickening agents, including thickener or gelling agents, includesubstances which that can increase the viscosity of a composition.Thickeners includes those that can increase the viscosity of acomposition without substantially modifying the efficacy of the activeingredient within the composition. Thickeners can also increase thestability of the compositions of the present invention. In certainaspects of the present invention, thickeners include hydrogenatedpolyisobutene or trihydroxystearin, or a mixture of both.

Non-limiting examples of additional thickening agents that can be usedin the context of the present invention include carboxylic acidpolymers, crosslinked polyacrylate polymers, polyacrylamide polymers,polysaccharides, and gums. Examples of carboxylic acid polymers includecrosslinked compounds containing one or more monomers derived fromacrylic acid, substituted acrylic acids, and salts and esters of theseacrylic acids and the substituted acrylic acids, wherein thecrosslinking agent contains two or more carbon-carbon double bonds andis derived from a polyhydric alcohol (see U.S. Pat. Nos. 5,087,445;4,509,949; 2,798,053; CTFA International Cosmetic Ingredient Dictionary,Fourth edition, 1991, pp. 12 and 80). Examples of commercially availablecarboxylic acid polymers include carbomers, which are homopolymers ofacrylic acid crosslinked with allyl ethers of sucrose or pentaerytritol(e.g., Carbopol™ 900 series from B. F. Goodrich).

Non-limiting examples of crosslinked polyacrylate polymers includecationic and nonionic polymers. Examples are described in U.S. Pat. Nos.5,100,660; 4,849,484; 4,835,206; 4,628,078; 4,599,379).

Non-limiting examples of polyacrylamide polymers (including nonionicpolyacrylamide polymers including substituted branched or unbranchedpolymers) include polyacrylamide, isoparaffin and laureth-7, multi-blockcopolymers of acrylamides and substituted acrylamides with acrylic acidsand substituted acrylic acids.

Non-limiting examples of polysaccharides include cellulose,carboxymethyl hydroxyethylcellulose, cellulose acetate propionatecarboxylate, hydroxyethylcellulose, hydroxyethyl ethylcellulose,hydroxypropylcellulose, hydroxypropyl methylcellulose, methylhydroxyethylcellulose, microcrystalline cellulose, sodium cellulosesulfate, and mixtures thereof. Another example is an alkyl substitutedcellulose where the hydroxy groups of the cellulose polymer ishydroxyalkylated (preferably hydroxy ethylated or hydroxypropylated) toform a hydroxyalkylated cellulose which is then further modified with aC₁₀-C₃₀ straight chain or branched chain alkyl group through an etherlinkage. Typically these polymers are ethers of C₁₀-C₃₀ straight orbranched chain alcohols with hydroxyalkylcelluloses. Other usefulpolysaccharides include scleroglucans comprising a linear chain of (1-3)linked glucose units with a (1-6) linked glucose every three unit.

Non-limiting examples of gums that can be used with the presentinvention include acacia, agar, algin, alginic acid, ammonium alginate,amylopectin, calcium alginate, calcium carrageenan, carnitine,carrageenan, dextrin, gelatin, gellan gum, guar gum, guarhydroxypropyltrimonium chloride, hectorite, hyaluroinic acid, hydratedsilica, hydroxypropyl chitosan, hydroxypropyl guar, karaya gum, kelp,locust bean gum, natto gum, potassium alginate, potassium carrageenan,propylene glycol alginate, sclerotium gum, sodium carboyxmethyl dextran,sodium carrageenan, tragacanth gum, xanthan gum, and mixtures thereof.

i. Preservatives

Non-limiting examples of preservatives that can be used in the contextof the present invention include quaternary ammonium preservatives suchas polyquaternium-1 and benzalkonium halides (e.g., benzalkoniumchloride (“BAC”) and benzalkonium bromide), parabens (e.g.,methylparabens and propylparabens), phenoxyethanol, benzyl alcohol,chlorobutanol, phenol, sorbic acid, thimerosal or combinations thereof.

2. Pharmaceutical Ingredients

Pharmaceutical active agents are also contemplated as being useful withthe compositions of the present invention. Non-limiting examples ofpharmaceutical active agents include anti-acne agents, agents used totreat rosacea, analgesics, anesthetics, anorectals, antihistamines,anti-inflammatory agents including non-steroidal anti-inflammatorydrugs, antibiotics, antifungals, antivirals, antimicrobials, anti-canceractives, scabicides, pediculicides, antineoplastics, antiperspirants,antipruritics, antipsoriatic agents, antiseborrheic agents, biologicallyactive proteins and peptides, burn treatment agents, cauterizing agents,depigmenting agents, depilatories, diaper rash treatment agents,enzymes, hair growth stimulants, hair growth retardants including DFMOand its salts and analogs, hemostatics, kerotolytics, canker soretreatment agents, cold sore treatment agents, dental and periodontaltreatment agents, photosensitizing actives, skin protectant/barrieragents, steroids including hormones and corticosteroids, sunburntreatment agents, sunscreens, transdermal actives, nasal actives,vaginal actives, wart treatment agents, wound treatment agents, woundhealing agents, etc.

G. Kits

Kits are also contemplated as being used in certain aspects of thepresent invention. For instance, compositions of the present inventioncan be included in a kit. A kit can include a container. Containers caninclude a bottle, a metal tube, a laminate tube, a plastic tube, adispenser, a pressurized container, a barrier container, a package, acompartment, a lipstick container, a compact container, cosmetic pansthat can hold cosmetic compositions, or other types of containers suchas injection or blow-molded plastic containers into which thedispersions or compositions or desired bottles, dispensers, or packagesare retained. The kit and/or container can include indicia on itssurface. The indicia, for example, can be a word, a phrase, anabbreviation, a picture, or a symbol.

The containers can dispense a pre-determined amount of the composition.In other embodiments, the container can be squeezed (e.g., metal,laminate, or plastic tube) to dispense a desired amount of thecomposition. The composition can be dispensed as a spray, an aerosol, aliquid, a fluid, or a semi-solid. The containers can have spray, pump,or squeeze mechanisms. A kit can also include instructions for employingthe kit components as well the use of any other compositions included inthe container. Instructions can include an explanation of how to apply,use, and maintain the compositions.

EXAMPLES

The following examples are included to demonstrate certain non-limitingaspects of the invention. It should be appreciated by those of skill inthe art that the techniques disclosed in the examples which followrepresent techniques discovered by the inventor to function well in thepractice of the invention. However, those of skill in the art should, inlight of the present disclosure, appreciate that many changes can bemade in the specific embodiments which are disclosed and still obtain alike or similar result without departing from the spirit and scope ofthe invention.

Example 1 Testing Vehicles

Non-limiting examples of compositions of the present invention aredescribed in Tables 1 and 2. These compositions can be used as vehiclesto test the efficacy of the active ingredients to treat skin.

TABLE 1* Ingredient % Concentration (by weight) Phase A Water q.s.Xanthum gum 0.1 M-paraben 0.15 P-paraben 0.1 Citric acid 0.01 Phase BCetyl alcohol 4.0 Glyceryl stearate + PEG 100 4.0 Octyl palmitate 4.0Dimethicone 1.0 Tocopheryl acetate 0.2 Phase C Active Ingredients** 2.0*Sprinkle Xanthum gum in water and mix for 10 min. Subsequently, add allingredients in phase A and heat to 70-75° C. Add all items in phase B toseparate beaker and heat to 70-75° C. Mix phases A and B at 70-75° C.Continue mixing and allow composition to cool to 30° C. Subsequently,add phase C ingredient while mixing. **Any of the active ingredients (orcombination thereof) described in the specification can be used. Forinstance, the active ingredients can include Argania spinosa (argan)seed kernel extract, Secale cereale (rye) seed extract, Linumusitatissimum (linseed) seed extract, Malpighia punicifolia (acerola)fruit extract, Terminalia ferdinandiana (kakadu plum) fruit extract,Myrciaria dubia (camu camu) fruit extract, Punica granatum(pomegrannate) fruit extract or sterols, Pinus sylvestris bark extract,Ribes nigrum (black currant) leaf extract, palmitoyl oligopeptide,palmitoyl tetrapeptide-7, or adenosine, or any combination thereof.Although the total amount of active ingredients in the Table 1formulation is 2% w/w, it is contemplated that the amount of activeingredients can be increased or decreased to achieve a desired result,where the water amount can be increased/decreased accordingly (e.g.,q.s.).

TABLE 2* Ingredient % Concentration (by weight) Phase A Water q.s.M-paraben 0.2 P-paraben 0.1 Na2 EDTA 0.1 Shea butter 4.5 Petrolatum 4.5Glycerin 4.0 Propylene Glycol 2.0 Finsolve TN 2.0 Phase B Sepigel 3052.0 Phase C Active Ingredient(s)** 2.0 *Add ingredients in phase A tobeaker and heat to 70-75° C. while mixing. Subsequently, add the phase Bingredient with phase A and cool to 30° C. with mixing. Subsequently,add phase C ingredient while mixing. **Any of the active ingredients (orcombination thereof) described in the specification can be used. Forinstance, the active ingredients can include Argania spinosa (argan)seed kernel extract, Secale cereale (rye) seed extract, Linumusitatissimum (linseed) seed extract, Malpighia punicifolia (acerola)fruit extract, Terminalia ferdinandiana (kakadu plum) fruit extract,Myrciaria dubia (camu camu) fruit extract, Punica granatum(pomegrannate) fruit extract or sterols, Pinus sylvestris bark extract,Ribes nigrum (black currant) leaf extract, palmitoyl oligopeptide,palmitoyl tetrapeptide-7, or adenosine, or any combination thereof.Although the total amount of active ingredients in the Table 1formulation is 2% w/w, it is contemplated that the amount of activeingredients can be increased or decreased to achieve a desired result,where the water amount can be increased/decreased accordingly (e.g.,q.s.).

Example 2 Non-Limiting Vehicles and Product Formulations

A non-limiting product formulation of the present invention is describedin Table 3.

TABLE 3* % Concentration Ingredient** (by weight) Malpighia punicifolia(acerola) extract 0.001 to 3% Argania spinosa (argan) extract 0.001 to3% Myrciaria dubia (camu camu) extract 0.001 to 3% Punica granatum(pomegrannate) extract 0.001 to 3% Pinus sylvestris extract 0.001 to 3%Terminalia ferdinandiana (kakadu plum) extract 0.001 to 3% Ribes nigrum(black current) leaf extract 0.001 to 3% dermatologically acceptablevehicle q.s. *Composition can be prepared by any known methods in theart. For instance, simple mixing of the plant extracts with adermatologically acceptable vehicle can be used. A non-limiting exampleof such a vehicle is illustrated in Table 4. **In particularembodiments, the plant extracts are Malpighia punicifolia (acerola)fruit extract; Argania spinosa (Argan) seed kernel extract; Myrciariadubia (Camu Camu) fruit extract; Punica granatum (pomegrannate) fruitextract or sterols; Pinus sylvestris bark extract; Terminaliaferdinandiana (Kakadu Plum) fruit extract; and Linum usitatissimum(linseed) seed extract. In other aspects, the following additional plantextracts can be added to the formulation in a concentration range of0.001 to 3%, by weight, of the total formulation: algae extract; yeastextract; Secale cereale (rye) seed extract; and Linum usitatissimum(linseed) extract.

A non-limiting dermatologically acceptable vehicle for the plantextracts of the present invention is described in Table 4. This base setof ingredients can be used to create emulsions, creams, lotions,solutions, etc. For instance, the Table 4 vehicle is used in the productformulation described in Table 5.

TABLE 4* % Concentration Ingredient (by weight) Water at least 60%Glycerin 3 to 10% Dimethicone or Cyclomethicone (or a mixture) 2 to 10%Hydrogenated polydecene 2 to 10% Butylene glycol 0.5 to 3%   Propyleneglycol 0.5 to 3%   Betaine 0.5 to 3%   additional cosmetic ingredientsq.s. *Composition can be prepared by any known methods in the art. Forinstance, simple mixing of the ingredients into a container can be used.Alternatively, the hydrophilic ingredients can be heated to 70-75° C.while mixing. Subsequently, the hydrophobic ingredients can be addedfollowed by any additional cosmetic ingredients with subsequent coolingof the mixture to 30° C. with mixing.

A non-limiting product formulation of the present invention is describedin Table 5. This formulation is an oil-in-water emulsion, the viscosityof which ranges from 30,000 to 50,000 cps, as measured on a BrookfieldViscometer using a TC spindle at 2.5 rpm at 25° C.

TABLE 5* % Concentration Ingredient (by weight) Water q.s. Glycerin5.135 Dimethicone 4.600 Hydrogenated Polydecene 4.500 Butylene Glycol1.600 Propylene Glycol 1.045 Betaine 1.000 Alcohol 0.520 PEG-32 0.500Maltodextrin 0.435 Polyacrylamide 0.400 Titanium Dioxide 0.400Triethanolamine 0.320 Diazolidinyl Urea 0.300 Acrylates/C10-30 AlkylAcrylate Cross Polymer 0.250 Alcohol Denat. 0.208 Disodium EDTA 0.201Argania Spinosa Kernel Extract 0.200 C13-14 Isoparaffin 0.200 MalpighiaPunicifolia (Aceroal) Fruit Extract 0.200 Urea 0.150 TerminalFerdinandiana Fruit Extract 0.127 Glucosamine HCL 0.125 Methylparaben0.1163 Algae Extract 0.100 Yeast Extract/Extrait De Levure 0.100Phenoxyethanol 0.095250 Lecithin 0.075 Silanetriol Trehalose Ether 0.070Myrciaria Dubia Fruit Extract 0.065 Sodium Cocoyl Glutamate 0.060 SecaleCereale (Rye) Seed Extract 0.060 Laureth-7 0.055 Xantham Gum 0.050Punical Granatum Extract 0.039 Propylparaben 0.032 GuarHydroxpropyltrimonium Chloride 0.020 Carbomer 0.011 Linum Usitatissimum(Linseed) Seed Extract 0.010 Pinus Sylvestris Bark Extract 0.0075 CetylHydroxyethylcellulose 0.004 Ribes Nigrum (Black Currant) Leaf Extract0.0025 Rutin 0.0025 Ethylparaben 0.002 Sulisobenzone 0.002 Chlorphenesin0.002 Sodium Methylparaben 0.0013 Butylparaben 0.001 PalmitoylOligopeptide 0.0002 Palmitoyl Tetrapeptide-7 0.0002 *Composition can beprepared by any known methods in the art. For instance, simple mixing ofthe ingredients into a container can be used. Alternatively, thehydrophilic ingredients can be heated to 70-75° C. while mixing.Subsequently, the Argania spinosa (Argan) seed kernel extract, Secalecereale (rye) seed extract, Linum usitatissimum (linseed) seed extract,Malpighia punicifolia (acerola) fruit extract, Terminalia ferdinandiana(Kakadu Plum) fruit extract, Myrciaria dubia (Camu Camu) fruit extract,Punica granatum (pomegrannate) fruit extract or sterols, Pinussylvestris bark extract, Ribes nigrum (black currant) leaf extract, canbe included with subsequent cooling of the mixture to 30° C. withmixing. The remaining ingredients can be added with continuous mixing.

Example 3 Supporting Data

Data supporting the efficacy of the following plant extracts ispresented in FIGS. 1-5: Argania spinosa (argan) seed kernel extract;Secale cereale (rye) seed extract; Linum usitatissimum (linseed) seedextract; Malpighia punicifolia (acerola) fruit extract; Terminaliaferdinandiana (kakadu plum) fruit extract; Myrciaria dubia (camu camu)fruit extract; Punica granatum (pomegrannate) fruit extract or sterols;Pinus sylvestris bark extract; and Ribes nigrum (black currant) leafextract. Note that the testing vehicle used to obtain these data is notdisclosed. However, these data can be confirmed by utilizing the testingvehicles described in Tables 1 and 2 of this application and theprocedures outlined in Example 4. These data confirm that the plantextracts stimulate synthesis of procollagen, elastin, FN and LM. Severalplant extracts inhibit the activity of multiple MMPs. Topicalapplication of a combination of plant-derived extracts stimulates matrixprotein synthesis in a tissue equivalent. Application of cosmeticformulation containing plant-derived extracts to human skin improvesfacial attributes as assessed by expert dermatological grader.Biomechanical characterization of skin following application offormulation containing plant extracts demonstrates improvement inpliability, softness and dryness.

Example 4 Determining Efficacy of the Compositions of the PresentInvention

The efficacy of compositions or active ingredients within a givencomposition of the present inventions can be determined by methods knownto those of ordinary skill in the art.

The following are non-limiting procedures that can be used in thecontext of the present invention. It should be recognized that othertesting procedures can be used, including, for example, objective andsubjective procedures. The active ingredients (e.g., Argania spinosa(argan) seed kernel extract, Secale cereale (rye) seed extract, Linumusitatissimum (linseed) seed extract, Malpighia punicifolia (acerola)fruit extract, Terminalia ferdinandiana (kakadu plum) fruit extract,Myrciaria dubia (camu camu) fruit extract, Punica granatum(pomegrannate) fruit extract or sterols, Pinus sylvestris bark extract,Ribes nigrum (black currant) leaf extract, palmitoyl oligopeptide,palmitoyl tetrapeptide-7, or adenosine, or any combination thereof) canbe tested for their skin efficacy by using the composition vehiclesidentified in Tables 1 and 2. As noted in these Tables, the activeingredients and concentration ranges can vary.

Skin moisture/hydration can be measured by using impedance measurementswith the Nova Dermal Phase Meter. The impedance meter measures changesin skin moisture content. The outer layer of the skin has distinctelectrical properties. When skin is dry it conducts electricity verypoorly. As it becomes more hydrated increasing conductivity results.Consequently, changes in skin impedance (related to conductivity) can beused to assess changes in skin hydration. The unit can be calibratedaccording to instrument instructions for each testing day. A notation oftemperature and relative humidity can also be made. Subjects can beevaluated as follows: prior to measurement they can equilibrate in aroom with defined humidity (e.g., 30-50%) and temperature (e.g.,68-72C). Three separate impedance readings can be taken on each side ofthe face, recorded, and averaged. The T5 setting can be used on theimpedance meter which averages the impedance values of every fiveseconds application to the face. Changes can be reported withstatistical variance and significance.

Skin clarity and the reduction in freckles and age spots can beevaluated using a Minolta Chromometer. Changes in skin color can beassessed to determine irritation potential due to product treatmentusing the a* values of the Minolta Chroma Meter. The a* value measureschanges in skin color in the red region. This is used to determinewhether a composition is inducing irritation. The measurements can bemade on each side of the face and averaged, as left and right facialvalues. Skin clarity can also be measured using the Minolta Meter. Themeasurement is a combination of the a*, b, and L values of the MinoltaMeter and is related to skin brightness, and correlates well with skinsmoothness and hydration. Skin reading is taken as above. In onenon-limiting aspect, skin clarity can be described as L/C where C ischroma and is defined as (a²+b²)^(1/2).

Skin dryness, surface fine lines, skin smoothness, and skin tone can beevaluated with clinical grading techniques. For example, clinicalgrading of skin dryness can be determined by a five point standardKligman Scale: (0) skin is soft and moist; (1) skin appears normal withno visible dryness; (2) skin feels slightly dry to the touch with novisible flaking; (3) skin feels dry, tough, and has a whitish appearancewith some scaling; and (4) skin feels very dry, rough, and has a whitishappearance with scaling. Evaluations can be made independently by twoclinicians and averaged.

Clinical grading of skin tone can be performed via a ten point analognumerical scale: (10) even skin of uniform, pinkish brown color. Nodark, erythremic, or scaly patches upon examination with a hand heldmagnifying lens. Microtexture of the skin very uniform upon touch; (7)even skin tone observed without magnification. No scaly areas, butslight discolorations either due to pigmentation or erythema. Nodiscolorations more than 1 cm in diameter; (4) both skin discolorationand uneven texture easily noticeable. Slight scaliness. Skin rough tothe touch in some areas; and (1) uneven skin coloration and texture.Numerous areas of scaliness and discoloration, either hypopigmented,erythremic or dark spots. Large areas of uneven color more than 1 cm indiameter. Evaluations were made independently by two clinicians andaveraged.

Clinical grading of skin smoothness can be analyzed via a ten pointanalog numerical scale: (10) smooth, skin is moist and glistening, noresistance upon dragging finger across surface; (7) somewhat smooth,slight resistance; (4) rough, visibly altered, friction upon rubbing;and (1) rough, flaky, uneven surface. Evaluations were madeindependently by two clinicians and averaged.

Skin smoothness and wrinkle reduction can also be assessed visually byusing the methods disclosed in Packman et al. (1978). For example, ateach subject visit, the depth, shallowness and the total number ofsuperficial facial lines (SFLs) of each subject can be carefully scoredand recorded. A numerical score was obtained by multiplying a numberfactor times a depth/width/length factor. Scores are obtained for theeye area and mouth area (left and right sides) and added together as thetotal wrinkle score.

Skin firmness can be measured using a Hargens ballistometer, a devicethat evaluates the elasticity and firmness of the skin by dropping asmall body onto the skin and recording its first two rebound peaks. Theballistometry is a small lightweight probe with a relatively blunt tip(4 square mm-contact area) was used. The probe penetrates slightly intothe skin and results in measurements that are dependent upon theproperties of the outer layers of the skin, including the stratumcorneum and outer epidermis and some of the dermal layers.

Skin softness/suppleness can be evaluated using the Gas BearingElectrodynamometer, an instrument that measures the stress/strainproperties of the skin. The viscoelastic properties of skin correlatewith skin moisturization. Measurements can be obtained on thepredetermined site on the cheek area by attaching the probe to the skinsurface with double-stick tape. A force of approximately 3.5 gm can beapplied parallel to the skin surface and the skin displacement isaccurately measured. Skin suppleness can then be calculated and isexpressed as DSR (Dynamic Spring Rate in gm/mm).

The appearance of lines and wrinkles on the skin can be evaluated usingreplicas, which is the impression of the skin's surface. Silicone rubberlike material can be used. The replica can be analyzed by imageanalysis. Changes in the visibility of lines and wrinkles can beobjectively quantified via the taking of silicon replicas form thesubjects' face and analyzing the replicas image using a computer imageanalysis system. Replicas can be taken from the eye area and the neckarea, and photographed with a digital camera using a low angle incidencelighting. The digital images can be analyzed with an image processingprogram and the are of the replicas covered by wrinkles or fine lineswas determined.

The surface contour of the skin can be measured by using theprofilometer/Stylus method. This includes either shining a light ordragging a stylus across the replica surface. The vertical displacementof the stylus can be fed into a computer via a distance transducer, andafter scanning a fixed length of replica a cross-sectional analysis ofskin profile can be generated as a two-dimensional curve. This scan canbe repeated any number of times along a fix axis to generate a simulated3-D picture of the skin. Ten random sections of the replicas using thestylus technique can be obtained and combined to generate averagevalues. The values of interest include Ra which is the arithmetic meanof all roughness (height) values computed by integrating the profileheight relative to the mean profile height. Rt which is the maximumvertical distance between the highest peak and lowest trough, and Rzwhich is the mean peak amplitude minus the mean peak height. Values aregiven as a calibrated value in mm. Equipment should be standardizedprior to each use by scanning metal standards of know values. Ra Valuecan be computed by the following equation: Ra=Standardize roughness;l_(m)=the traverse (scan) length; and y=the absolute value of thelocation of the profile relative to the mean profile height (x-axis).

In other non-limiting aspects, the efficacy of the compositions of thepresent invention can be evaluated by using a skin analog, such as, forexample, MELANODERM™. Melanocytes, one of the cells in the skin analog,stain positively when exposed to L-dihydroxyphenyl alanine (L-DOPA), aprecursor of melanin. The skin analog, MELANODERM™, can be treated witha variety of bases containing the compositions and whitening agents ofthe present invention or with the base alone as a control.Alternatively, an untreated sample of the skin analog can be used as acontrol.

All of the compositions and/or methods disclosed and claimed in thisspecification can be made and executed without undue experimentation inlight of the present disclosure. While the compositions and methods ofthis invention have been described in terms of particular embodiments,it will be apparent to those of skill in the art that variations may beapplied to the compositions and/or methods and in the steps or in thesequence of steps of the method described herein without departing fromthe concept, spirit and scope of the invention. More specifically, itwill be apparent that certain agents which are both chemically andphysiologically related may be substituted for the agents describedherein while the same or similar results would be achieved. All suchsimilar substitutes and modifications apparent to those skilled in theart are deemed to be within the spirit, scope and concept of theinvention as defined by the appended claims.

1. A topical skin care composition comprising: (a) a combination of thefollowing extracts: Malpighia punicifolia (acerola) extract; Arganiaspinosa (argan) extract; Myrciaria dubia (camu camu) extract; Punicagranatum (pomegrannate) extract; Pinus sylvestris extract; Terminaliaferdinandiana (kakadu plum) extract; Linum usitatissimum (linseed)extract; Ribes nigrum (black current) extract; Secale cereale (rye)extract; algae extract; and yeast extract; and (b) a dermatologicallyacceptable vehicle.
 2. The topical skin care composition of claim 1,wherein the dermatologically acceptable vehicle comprises: (i) water;(ii) glycerin; (iii) dimethicone or cyclomethicone; (iv) hydrogenatedpolydecene; (v) butylene glycol; (vi) propylene glycol; and (vii)betaine.
 3. The topical skin care composition of claim 2, wherein thedermatologically acceptable vehicle comprises: (i) at least 60% byweight of water; (ii) 3 to 10% by weight of glycerin; (iii) 2 to 10% byweight of dimethicone or cyclomethicone; (iv) 2 to 10% by weight ofhydrogenated polydecene; (v) 0.5 to 3% by weight of butylene glycol;(vi) 0.5 to 3% by weight of propylene glycol; and (vii) 0.5 to 3% byweight of betaine.
 4. The topical skin care composition of claim 3,wherein the composition includes 0.5 to 3.0% by weight of thecombination of extracts.
 5. The topical skin care composition of claim5, wherein the composition has a viscosity ranging from 30,000 to 50,000cps as measured by a Brookfield Viscometer using a TC spindle at 2.5 rpmat 25° C.
 6. The topical skin care composition of claim 5, wherein thecomposition has a an oxygen radical absorbance capacity (ORAC) value permg of at least about 5.0 to about 25.0.
 7. The topical skin carecomposition of claim 1, wherein the composition has the formuladescribed at Table
 5. 8. The topical skin care composition of claim 1,wherein the composition consists of the formula described at Table
 5. 9.The topical skin care composition of claim 1, wherein the composition isformulated as an oil-in-water emulsion.
 10. The topical skin carecomposition of claim 1, wherein the composition is formulated as aserum.
 11. A method of reducing the appearance of a skin conditioncomprising topically applying the composition of claim 1 to the skincondition, wherein topical application of the composition to skincondition reduces the appearance of the skin condition.
 12. The methodof claim 11, wherein the skin condition is a fine line or wrinkle. 13.The method of claim 11, wherein the skin condition is uneven skin toneor an age spot.
 14. The method of claim 11, wherein the skin conditionis located on facial skin.
 15. A method of increasing the firmness ofskin comprising topically applying the composition of claim 1 to skin inneed thereof, wherein topical application of the composition to skinincreases the firmness of skin.
 16. The method of claim 15, wherein theskin in need thereof comprises a fine line or wrinkle.
 17. The method ofclaim 16, wherein the composition is applied to facial skin.
 18. Amethod of increasing collagen, fibronectin, or laminin production in askin cell comprising topically applying the composition of claim 1 to askin cell that is in need of collagen, fibronectin, or lamininproduction, wherein the topical application of the composition to theskin cell increases collagen, fibronectin, or laminin production in theskin cell.
 19. The method of claim 18, wherein collagen, fibronectin,and laminin production are increased in the skin cell.